CNE1-Luc cell line

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CNE1-Luc (Hela-contaminated cells, not for sales) Order

Catalog#

YC-C062-Luc-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Luciferase Stable Cell Lines

cell name

CNE1-Luc

Morphology

Endothelial cells, adherent

Passage ratio

1:3~1:5

Species

Human

Reporter gene

Luciferase

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:CNE1			STR Info (Cell bank) Cell Line:CNE-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	10	12	13.3	10	12	13.3
D7S820	10	12		10	12
D16S539	9	10		9	10
VWA	14	16		14	16
TH01	6	7	9.0	6	7	9.0
AMEL	X	X		X	X
TPOX	8	12		8	12
CSF1PO	10	11		10	11

* Hela-contaminated cells, not for sales

Reviewed by ICLAC (ref: 121113, 150506). STR profiling in Chan et al (2008) demonstrated similarity to HeLa, but at less than 80% match. Strong et al (2014) presented additional data in support of this finding, demonstrating that cells carry HPV-18, as previously demonstrated in HeLa cells. The authors speculate that a fusion event may have occurred between HeLa and an unknown cell line; although not proven, current data are consistent with this hypothesis.

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the CNE1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a CNE1-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
01
Sensitive accurate：Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
02
High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
03

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